Isolated anaerobic bacteria were found ...

Doan M, B Baysal

Karaman Devlet Hastanesi, Mikrobiyoloji Laboratuvari, Karaman. metin_dogan42 @ Yahoo. com

Mikrobiyol Bul 2010Apr, 44 (2)

:211-9. Conventional separation, identification and determination of susceptibility of anaerobic bacteria present with a number of difficulties that lead to defects in determining the susceptibility of local features that will guide empirical treatment protocols. This study was conducted to identify anaerobic bacteria isolated from different clinical materials obtained from patients with suspicion of anaerobic infection and determination of antibiotic susceptibility to multiple antibiotics. One hundred clinical samples (36 blood, 31 abscess, 12 peritoneal fluid, joint fluid 7, 7 pleural fluid, 3 biopsy, 3 cerebrospinal fluid and a surgical wound), which were examined in our laboratory for 20 March to 30 October 2007, were included in the study. Samples were collected and transported under anaerobic conditions and sown in conventional aerobic media and Wilkins Chalgren agar, Schaedler agar and chopped meat broth for anaerobic isolation. Isolated anaerobic bacteria were detected with the API panel 20A (Bio-Mere, France) using traditional methods and using the AN-IDENT discs (Oxoid, England). Penicillin G, clindamycin, cefoxitin, metronidazole, piperacillin / tazobaktam and imipenem susceptibility tests were carried out with the E-method. Twenty-two anaerobic bacteria were isolated from 14 clinical samples, 7 samples of strattera no prescritpion growth brings more than one type of anaerobic bacteria and 8 samples brings both anaerobic and facultative anaerobic bacteria (4 E. coli and 4 Enterococcus spp.) Growth. Anaerobic bacteria were isolated in 89 abscess and 6 samples of peritoneal fluid. Distribution of anaerobic bacteria to identify among these samples were as follows: Bacteroides fragile (n = 6), Bacteroides spp. except B. fragile (n = 4), Clostridium SPP. (L = 2), Fusobacterium necrophorum / nucleatum (n = 1), Prevotella intermediate / disiens (n ​​= 1), Peptococcus Niger (n = 2), Peptostreptococcus SPP. (L = 5) and Lactobacillus acidophilus / lenseii (n = 1). Beta-lactamase activity was detected only in 2 of 6 strains of B. brittle. All strains were susceptible to imipenem and piperacillin / tazobaktam. The highest level of resistance was found to penicillin G (9/22, 41%). While anaerobic gram-positive cocci (n = 7) were sensitive to all antibiotics, the rate of resistance among gram-negative anaerobic bacteria was 75% (9/12) to penicillin, 33. 3% (4/12) to clindamycin, 8. 3% (1/12) metronidazole. Among the anaerobic gram-positive bacteria (n = 3), 2 were resistant to metronidazole, clindamycin and one to a cefoxitin. The results of this first study of anaerobic antimicrobial susceptibility testing performed in Konya in Turkey showed that penicillin was not necessary in the empirical treatment of anaerobic infections, clindamycin susceptibility should be tested before use, metronidazole and cefoxitin can be used in empirical therapy and imipenem and piperacillin / tazobaktam should be reserved for treatment of complicated infections and infections caused by resistant bacteria. .